Vitamin B12 10ml

What is Vitamin B12 10ml?

Vitamin B12 10ml is a 10 ml multi-dose research solution of cobalamin, the cobalt-containing corrinoid vitamin that serves as coenzyme for methionine synthase and methylmalonyl-CoA mutase in mammalian cells. It is supplied as methylcobalamin (CAS 13422-55-4, C63H91CoN13O14P, 1344.4 g/mol) or cyanocobalamin (CAS 68-19-9, C63H88CoN14O14P, 1355.4 g/mol), with the form present stated on the batch Certificate of Analysis. Vitamin B12 is not a peptide; the deep red colour of the solution comes from the corrin ring around the cobalt centre.

GenoPept supplies the vitamin B12 research solution to UK laboratories that need a defined cobalamin source for cell culture, enzymology and animal-model work, and as a companion to lipotropic solutions such as Lipo-B that include cobalamin in a mixture.

Vitamin B12 10ml research applications

One-carbon and homocysteine metabolism

Vitamin B12 is the cofactor that lets methionine synthase remethylate homocysteine using 5-methyltetrahydrofolate. Research uses defined cobalamin supply to study homocysteine accumulation, folate trapping and S-adenosylmethionine levels.

DNA and histone methylation

Because methionine feeds S-adenosylmethionine, vitamin B12 status is investigated as a variable in epigenetic methylation studies in cultured cells and rodent tissue.

Haematopoiesis models

Cobalamin-deficient and replete conditions are compared in erythroid cell lines and animal models of megaloblastic change.

Neuronal and myelin research

Methylcobalamin in particular is studied in neuronal cultures and nerve-injury models for effects on axonal outgrowth and myelin-related gene expression.

Defined culture media and analytics

Vitamin B12 is a standard component of serum-free media and a reference standard in LC-MS cobalamin assays.

Mechanism of action (preclinical)

Vitamin B12 acts as a coenzyme for two mammalian enzymes. Methylcobalamin is the cofactor of cytosolic methionine synthase, which transfers a methyl group from 5-methyltetrahydrofolate to homocysteine to regenerate methionine and tetrahydrofolate, linking the folate cycle to the methionine cycle and S-adenosylmethionine synthesis.

Adenosylcobalamin is the cofactor of mitochondrial methylmalonyl-CoA mutase, which converts methylmalonyl-CoA to succinyl-CoA in the catabolism of odd-chain fatty acids and several amino acids. Cyanocobalamin supplied to cells is reduced and converted to these coenzyme forms intracellularly. Cellular uptake in preclinical models proceeds through transcobalamin-mediated endocytosis, and both coenzyme forms are studied in cobalamin-deficiency systems.

Vitamin B12 10ml product specifications

Available sizes

• Vitamin B12 10 ml multi-dose research solution vial

Vitamin B12 is supplied in a single 10 ml vial size from UK stock. Boxes of 10 vials save 20 to 50% through the bulk and wholesale page.

Reconstitution, handling and storage (laboratory)

Vitamin B12 is supplied as a 10 ml multi-dose research solution and does not require reconstitution. Store unopened vials at 2 to 8 °C, protected from light, and do not freeze; freezing can cause components to precipitate. Allow the vial to reach room temperature before drawing so that volumes are accurate.

Swab the stopper before each draw and use a fresh sterile needle every time to protect the multi-dose seal. Record the first-puncture date on the label, return the vial to the refrigerator between uses, and discard it at the end of the period set by your protocol or if the solution changes colour, turns cloudy or shows particulates. Methylcobalamin is especially light-sensitive, so keep the vial in its carton or an amber holder. General guidance is in the storage guide on the site.

Quality assurance and Certificate of Analysis

Every batch of Vitamin B12 10ml supplied by GenoPept is tested by an independent UK third-party laboratory (Puralytix, ISO/IEC 17025 accredited). Testing confirms the cobalamin form present by LC-MS and its purity by HPLC. The batch Certificate of Analysis (COA) is available through the “View Certificate of Analysis” button on this page, and the full library is published at genopept.co.uk/coa-certificates.

Each vial is sealed, batch-identified with lot number and expiry date, and dispatched by tracked Royal Mail Special Delivery within the UK (free on orders of £200 or more). Read what a Certificate of Analysis shows to interpret the report.

Related research compounds

• Lipo-B 10ml: a lipotropic solution that includes vitamin B12 in a combined formulation.
• Lipo-C Fat Metabolism Complex 10ml: a lipotropic and B-vitamin complex for pathway comparison.
• NAD+: a redox cofactor studied in the same energy-metabolism models.
• Glutathione (GSH): the thiol antioxidant linked to one-carbon metabolism through the transsulfuration pathway.

Vitamin B12 10ml frequently asked questions

What is vitamin B12 used for in research?

Vitamin B12 is used in research as the cobalamin cofactor for methionine synthase and methylmalonyl-CoA mutase. Laboratories use the solution in cell-culture and animal models of one-carbon metabolism, homocysteine remethylation, DNA methylation, red-cell development and neuronal myelination, and as a supplement in defined culture media. It is supplied strictly for in-vitro laboratory research.

What is the difference between methylcobalamin and cyanocobalamin?

Methylcobalamin is the methylated coenzyme form of cobalamin that methionine synthase uses directly, while cyanocobalamin is a stable synthetic form with a cyanide ligand that cells convert to the active coenzymes. Both share the same corrin ring and cobalt centre. Research often compares them for stability, cellular uptake and conversion efficiency in model systems.

How is vitamin B12 supplied and verified?

GenoPept supplies vitamin B12 as a 10 ml multi-dose red liquid research solution in a sealed, batch-identified glass vial. The cobalamin form present (methylcobalamin or cyanocobalamin) and its purity are confirmed for each batch by an independent ISO/IEC 17025 laboratory using LC-MS identity and HPLC purity testing, with the Certificate of Analysis linked from this page.

How should vitamin B12 solution be stored in the laboratory?

Store the vitamin B12 solution at 2 to 8 °C, protected from light, and do not freeze. Cobalamins, and methylcobalamin in particular, are light-sensitive, so keep the vial in its carton or an amber holder at all times. After the first puncture, date the label, swab the stopper and use a fresh sterile needle for each draw. Discard if the red colour fades or the solution turns cloudy.

Is vitamin B12 from GenoPept for human use?

No. The vitamin B12 research solution is sold strictly for in-vitro laboratory research. It is not a medicine, supplement or injectable product, is not for human or veterinary use, and is not intended for diagnostic or therapeutic use. Purchasers confirm they are buying for research and accept the research use only terms before ordering.

Research use only. This product is supplied for in-vitro laboratory research only. It is not for human or veterinary use, is not a medicine, and is not intended for diagnostic or therapeutic use. See the research use only policy for full terms.